Optimized Condition for Enhanced Soluble-Expression of Recombinant Mutant Anabaena Variabilis Phenylalanine Ammonia Lyase

• Main Authors: Hossein Zarei Jaliani (Author), Safar Farajnia (Author), Yaghoub Safdari (Author), Seyyed Abolghasem Mohammadi (Author), Abolfazl Barzegar (Author), Saeed Talebi (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2014-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Recently discovered Anabaena variabilis phenylalanine ammonia lyase (AvPAL) proved to be a good candidate for enzyme replacement therapy of phenylketonuria. Outstanding stability properties of a mutant version of this enzyme, produced already in our laboratory, have led us to the idea of cu...