A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery

Purpose: To develop a simple method to extract the whole apolipoproteins (apo) including apoA-I in native high density lipoproteins (HDLs) and prepare discoidal Tanshinone IIA-loaded reconstituted HDL (TA-rHDLs) as a dual functional drug delivery system with plaque-site target and therapeutic promis...

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Main Authors: Wenli Zhang (Author), Ji Wang (Author), Junting Jia (Author), Liang Chen (Author), Zimei Wu (Author), Jianping Liu (Author)
Format: Book
Published: Frontiers Media S.A., 2015-05-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Wenli Zhang  |e author 
700 1 0 |a Ji Wang  |e author 
700 1 0 |a Junting Jia  |e author 
700 1 0 |a Liang Chen  |e author 
700 1 0 |a Zimei Wu  |e author 
700 1 0 |a Jianping Liu  |e author 
245 0 0 |a A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery 
260 |b Frontiers Media S.A.,   |c 2015-05-01T00:00:00Z. 
500 |a 10.18433/J3531X 
500 |a 1482-1826 
520 |a Purpose: To develop a simple method to extract the whole apolipoproteins (apo) including apoA-I in native high density lipoproteins (HDLs) and prepare discoidal Tanshinone IIA-loaded reconstituted HDL (TA-rHDLs) as a dual functional drug delivery system with plaque-site target and therapeutic promises in atherosclerotic lesions. Methods: A method based on isoelectric precipitation coupled with organic solvent precipitation was developed to isolate the whole apolipoproteins (apos). TA-rHDLs were prepared by incubating the resultant apos with liposomes and the incubation conditions were optimized using fluorescence quenching experiment. TA-rHDLs were characterized in terms of size, zeta potential, morphology, interaction between lipid and apos,  safety, and bionic function. Results: The extraction results showed that the yield of the HDL apos was 82.4%, with 59% being apoA-I type, similar ratio of apoA-I in the native apos. TA-rHDL prepared were disc-like with an average diameter of 157.6 ± 4.8 nm, zeta potential of -20.90 ± 0.15 mV, and entrapment efficiency of (90.13 ± 1.4) %. The interaction between the lipids and apos was electrostatic and hydrophobic force and was associated with amino acid sequence. Haemolysis and cytotoxicity assays showed good biocompatibility of TA-rHDL. Sterol efflux assay from macrophages mediated by TA-rHDLs and structure remodeling behavior from discs to spheres proved that TA-rHDL could resemble the biological activity of native nascent HDL irrespective of the size. Conclusions: The simple approach to isolate apos may provide a convenient and economical resource to support the development of rHDL as a potential targeting nanocarrier for lipophilic cardiovascular drugs. This article is open to POST-PUBLICATION REVIEW. Registered readers (see "For Readers") may comment by clicking on ABSTRACT on the issue's contents page. 
546 |a EN 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
690 |a Pharmacy and materia medica 
690 |a RS1-441 
655 7 |a article  |2 local 
786 0 |n Journal of Pharmacy & Pharmaceutical Sciences, Vol 18, Iss 2 (2015) 
787 0 |n https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/24334 
787 0 |n https://doaj.org/toc/1482-1826 
856 4 1 |u https://doaj.org/article/adf1a8d98b504495934cbc0bbae1cf5c  |z Connect to this object online.