Diurnal Rhythmicity in the Transcription of Jejunal Drug Transporters†
Intestinal drug efflux proteins play a major role in the pharmacokinetics of many drugs. We assessed diurnal rhythmicity in the expression of ten major drug transporters. We acquired male Sprague-Dawley rats and harvested jejunal mucosa at 3-h intervals across a 24-h period. qPCR was performed for t...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Book |
| Published: |
Elsevier,
2008-01-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_ae85e3d68e51418caf34b9c736d25b4f | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Adam T. Stearns |e author |
| 700 | 1 | 0 | |a Anita Balakrishnan |e author |
| 700 | 1 | 0 | |a David B. Rhoads |e author |
| 700 | 1 | 0 | |a Stanley W. Ashley |e author |
| 700 | 1 | 0 | |a Ali Tavakkolizadeh |e author |
| 245 | 0 | 0 | |a Diurnal Rhythmicity in the Transcription of Jejunal Drug Transporters† |
| 260 | |b Elsevier, |c 2008-01-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1254/jphs.08100SC | ||
| 520 | |a Intestinal drug efflux proteins play a major role in the pharmacokinetics of many drugs. We assessed diurnal rhythmicity in the expression of ten major drug transporters. We acquired male Sprague-Dawley rats and harvested jejunal mucosa at 3-h intervals across a 24-h period. qPCR was performed for ten transporters: Mdr1, Mdr3, Mrp1 - 3, Mct1, Brcp, Pept1, Octn2, and Oatp-b. Rhythmicity was assessed with the cosinor procedure. Diurnal rhythmicity was observed for Mdr1, Mct1, Mrp2, Pept1, and Bcrp (1.6 - 5.4-fold-changes). Acrophases occurred during fasting hours. We conclude that many drug transporters display profound diurnal rhythms in transcription, which may underlie diurnal rhythms in drug pharmacokinetics. Keywords:: diurnal rhythm, intestinal, drug efflux transporter | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 108, Iss 1, Pp 144-148 (2008) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861319313866 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/ae85e3d68e51418caf34b9c736d25b4f |z Connect to this object online. |