A FRET flow cytometry method for monitoring cytosolic and glycosomal glucose in living kinetoplastid parasites.

• Glavni autori: Charles M Voyton (Autor), Yijian Qiu (Autor), Meredith T Morris (Autor), P Christine Ackroyd (Autor), Jimmy Suryadi (Autor), Logan Crowe (Autor), James C Morris (Autor), Kenneth A Christensen (Autor)
• Format: Knjiga
• Izdano: Public Library of Science (PLoS), 2018-05-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

The bloodstream lifecycle stage of the kinetoplastid parasite Trypanosoma brucei relies solely on glucose metabolism for ATP production, which occurs in peroxisome-like organelles (glycosomes). Many studies have been conducted on glucose uptake and metabolism, but none thus far have been able to mon...