A FRET flow cytometry method for monitoring cytosolic and glycosomal glucose in living kinetoplastid parasites.

• Main Authors: Charles M Voyton (Author), Yijian Qiu (Author), Meredith T Morris (Author), P Christine Ackroyd (Author), Jimmy Suryadi (Author), Logan Crowe (Author), James C Morris (Author), Kenneth A Christensen (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The bloodstream lifecycle stage of the kinetoplastid parasite Trypanosoma brucei relies solely on glucose metabolism for ATP production, which occurs in peroxisome-like organelles (glycosomes). Many studies have been conducted on glucose uptake and metabolism, but none thus far have been able to mon...