A FRET flow cytometry method for monitoring cytosolic and glycosomal glucose in living kinetoplastid parasites.

• Hauptverfasser: Charles M Voyton (Verfasst von), Yijian Qiu (Verfasst von), Meredith T Morris (Verfasst von), P Christine Ackroyd (Verfasst von), Jimmy Suryadi (Verfasst von), Logan Crowe (Verfasst von), James C Morris (Verfasst von), Kenneth A Christensen (Verfasst von)
• Format: Buch
• Veröffentlicht: Public Library of Science (PLoS), 2018-05-01T00:00:00Z.
• Schlagworte: article
• Online-Zugang: Connect to this object online.

The bloodstream lifecycle stage of the kinetoplastid parasite Trypanosoma brucei relies solely on glucose metabolism for ATP production, which occurs in peroxisome-like organelles (glycosomes). Many studies have been conducted on glucose uptake and metabolism, but none thus far have been able to mon...