Contribution of Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells to Chimeras through Injection and Coculture of Embryos

• Main Authors: Jitong Guo (Author), Baojiang Wu (Author), Shuyu Li (Author), Siqin Bao (Author), Lixia Zhao (Author), Shuxiang Hu (Author), Wei Sun (Author), Jie Su (Author), Yanfeng Dai (Author), Xihe Li (Author)
• Format: Book
• Published: Hindawi Limited, 2014-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Blastocyst injection and morula aggregation are commonly used to evaluate stem cell pluripotency based on chimeric contribution of the stem cells. To assess the protocols for generating chimeras from stem cells, 8-cell mouse embryos were either injected or cocultured with mouse embryonic stem cells...