Single-reaction, multiplex, real-time rt-PCR for the detection, quantitation, and serotyping of dengue viruses.

• Main Authors: Jesse J Waggoner (Author), Janaki Abeynayake (Author), Malaya K Sahoo (Author), Lionel Gresh (Author), Yolanda Tellez (Author), Karla Gonzalez (Author), Gabriela Ballesteros (Author), Anna M Pierro (Author), Paolo Gaibani (Author), Frances P Guo (Author), Vittorio Sambri (Author), Angel Balmaseda (Author), Kumudu Karunaratne (Author), Eva Harris (Author), Benjamin A Pinsky (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-P...