Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA

• Main Authors: Morten Lundh (Author), Kaja Pluciñska (Author), Marie S. Isidor (Author), Patricia S.S. Petersen (Author), Brice Emanuelli (Author)
• Format: Bog
• Udgivet: Elsevier, 2017-10-01T00:00:00Z.
• Fag: article
• Online adgang: Connect to this object online.

Objective: Functional investigation of novel gene/protein targets associated with adipocyte differentiation or function heavily relies on efficient and accessible tools to manipulate gene expression in adipocytes in vitro. Recent advances in gene-editing technologies such as CRISPR-Cas9 have not onl...