Oocyte activation with phospholipase Cζ mRNA induces repetitive intracellular Ca2+ rises and improves the quality of pig embryos after intracytoplasmic sperm injection

• Main Authors: Michiko NAKAI (Author), Shun-ichi SUZUKI (Author), Dai-ichiro FUCHIMOTO (Author), Shoichiro SEMBON (Author), Kazuhiro KIKUCHI (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2024-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

For the intracytoplasmic sperm injection (ICSI) procedure in pigs, an electrical pulse (EP) has been used as an effective method for oocyte stimulation, but unlike sperm, EP is unable to induce Ca2+ oscillations. In this study, we investigated the effects of generating artificial Ca2+ oscillations w...