Non-targeting control for MISSION shRNA library silences SNRPD3 leading to cell death or permanent growth arrest

• Main Authors: Maria Czarnek (Author), Katarzyna Sarad (Author), Agnieszka Karaś (Author), Jakub Kochan (Author), Joanna Bereta (Author)
• Format: Bog
• Udgivet: Elsevier, 2021-12-01T00:00:00Z.
• Fag: article
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In parallel with the expansion of RNA interference (RNAi) techniques, accumulating evidence indicates that RNAi analyses might be seriously biased due to the off-target effects of gene-specific short hairpin RNAs (shRNAs). Our findings indicated that off-target effects of non-targeting shRNA compris...