Diagnosis of Genus Helicobacter through a hemi-nested PCR assay o

• Main Authors: Heping Qin (Author), Guodu Tang (Author), Ping Yi (Author), Xinyi Pan (Author), Huali Huang (Author), Renjie Chang (Author), Zhe Shi (Author), Muhammad Aqeel Ashraf (Author)
• Format: Book
• Published: Elsevier, 2016-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The present study aimed to establish a genus-specific PCR-based assay to detect helicobacters using 16S rRNA gene as the target template. We designed the hemi-nested primers based on sequences of 16S rRNA gene of 34 types of Helicobacter species. The inclusivity, sensitivity, and specificity of the...