Convenience versus Biological Significance: Are PMA-Differentiated THP-1 Cells a Reliable Substitute for Blood-Derived Macrophages When Studying in Vitro Polarization?

• Main Authors: Serena Tedesco (Author), Federica De Majo (Author), Jieun Kim (Author), Annalisa Trenti (Author), Lucia Trevisi (Author), Gian Paolo Fadini (Author), Chiara Bolego (Author), Peter W. Zandstra (Author), Andrea Cignarella (Author), Libero Vitiello (Author)
• Format: Book
• Published: Frontiers Media S.A., 2018-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Human peripheral-blood monocytes are used as an established in vitro system for generating macrophages. For several reasons, monocytic cell lines such as THP-1 have been considered as a possible alternative. In view of their distinct developmental origins and phenotypic attributes, we set out to ass...