Phase II evaluation of sensitivity and specificity of PCR and NASBA followed by oligochromatography for diagnosis of human African trypanosomiasis in clinical samples from D.R. Congo and Uganda.

• Main Authors: Enock Matovu (Author), Claire M Mugasa (Author), Rosine Ali Ekangu (Author), Stijn Deborggraeve (Author), George W Lubega (Author), Thierry Laurent (Author), Gerard J Schoone (Author), Henk D Schallig (Author), Philippe Büscher (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2010-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND: The polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA) have been recently modified by coupling to oligochromatography (OC) for easy and fast visualisation of products. In this study we evaluate the sensitivity and specificity of the PCR-OC and NASBA-OC...