Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples.

• Main Authors: Carolina I Cura (Author), Tomas Duffy (Author), Raúl H Lucero (Author), Margarita Bisio (Author), Julie Péneau (Author), Matilde Jimenez-Coello (Author), Eva Calabuig (Author), María J Gimenez (Author), Edward Valencia Ayala (Author), Sonia A Kjos (Author), José Santalla (Author), Susan M Mahaney (Author), Nelly M Cayo (Author), Claudia Nagel (Author), Laura Barcán (Author), Edith S Málaga Machaca (Author), Karla Y Acosta Viana (Author), Laurent Brutus (Author), Susana B Ocampo (Author), Christine Aznar (Author), Cesar A Cuba Cuba (Author), Ricardo E Gürtler (Author), Janine M Ramsey (Author), Isabela Ribeiro (Author), John L VandeBerg (Author), Zaida E Yadon (Author), Antonio Osuna (Author), Alejandro G Schijman (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2015-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI-TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have been developed with variable levels of complexity, selectivit...