Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos

• Main Authors: Weikai Ge (Author), Xiaozhu Zhao (Author), Shixue Gou (Author), Qin Jin (Author), Fangbing Chen (Author), Zhen Ouyang (Author), Chengdan Lai (Author), Tao Cui (Author), Baoyi Mai (Author), Sijia Lu (Author), Kexin Zhong (Author), Yanhui Liang (Author), Tao Chen (Author), Han Wu (Author), Nan Li (Author), Yinghua Ye (Author), Liangxue Lai (Author), Kepin Wang (Author)
• Format: Book
• Published: Elsevier, 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Cas9 protein without sgRNAs can induce genomic damage at the cellular level in vitro. However, whether the detrimental effects occur in embryos after Cas9 treatment remains unknown. Here, using pig embryos as subjects, we observed that Cas9 protein transcribed from injected Cas9 mRNA can persist unt...