Rapid and Sensitive Identification of Bacterial Infection and Bacteria Gram Types in Pleural Fluid of Children

• Main Authors: Yi-Dong Wu PhD (Author), Wei Li MD (Author), Yi Wei MD (Author), Hui-Hui Gao PhD (Author), Shi-Qiang Shang MD (Author), Li-Zhong Du PhD (Author)
• Format: Book
• Published: SAGE Publishing, 2015-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Real-time polymerase chain reaction (RT-PCR) techniques have been increasingly used to detect microbial DNA in clinic for the diagnosis of bacterial infection. This study aims to developing an RT-PCR method to detect bacteria in pleural fluid (PF). We performed a method to simultaneously detect and...