A Simple and Efficient In Vivo non-viral RNA Transfection Method For Labeling the Whole Axonal Tree of Individual Adult Long-Range Projection Neurons

• Main Authors: César ePorrero (Author), Javier eRodríguez-Moreno (Author), José I Quetglas (Author), Cristian eSmerdou (Author), Takahiro eFuruta (Author), Francisco eClasca (Author)
• Format: Book
• Published: Frontiers Media S.A., 2016-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We report a highly efficient, simple and non-infective method for labeling individual long-range projection neurons in a specific location with enough sparseness and intensity to allow complete and unambiguous reconstructions of their entire axonal tree. The method is based on the in vivo transfecti...