Enzyme immunoassays (EIA) for serodiagnosis of human leptospirosis: specific IgG3/IgG1 isotyping may further inform diagnosis of acute disease.

• Main Authors: Elsa Fortes-Gabriel (Author), Mariana Soares Guedes (Author), Advait Shetty (Author), Charles Klazer Gomes (Author), Teresa Carreira (Author), Maria Luísa Vieira (Author), Lisa Esteves (Author), Luísa Mota-Vieira (Author), Maria Gomes-Solecki (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2022-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The laborious microscopic agglutination test (MAT) is the gold standard serologic test for laboratory diagnosis of leptospirosis. We developed EIA based serologic assays using recombinant proteins (rLigA, rLigB, rLipL32) and whole-cell extracts from eight Leptospira serovars as antigen and assessed...