Cloning and Overexpression of Strictosidine β-D-Glucosidase Gene Short Sequence from Catharanthus roseus in Escherichia coli

• Main Authors: Ahmed Saeed Arafa (Author), Amany Elsayed Ragab (Author), Abdel-Rahim Sayed Ibrahim (Author), Wael Saad Abdel-Mageed (Author), Mahmoud Emam Nasr (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2019-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Strictosidine-β-D-glucosidase (SGD) is considered as a key enzyme in the production of bisindole alkaloids in Catharanthus roseus. The present study illustrated the production of a short sequence of this enzyme in Escherichia coli without codon optimization. Methods: Strictosidine-β-D-gluco...