Cloning, purification, kinetic and anion inhibition studies of a recombinant β-carbonic anhydrase from the Atlantic salmon parasite platyhelminth Gyrodactylus salaris

• Main Authors: Ashok Aspatwar (Author), Harlan Barker (Author), Heidi Aisala (Author), Ksenia Zueva (Author), Marianne Kuuslahti (Author), Martti Tolvanen (Author), Craig R. Primmer (Author), Jaakko Lumme (Author), Alessandro Bonardi (Author), Amit Tripathi (Author), Seppo Parkkila (Author), Claudiu T. Supuran (Author)
• Format: Book
• Published: Taylor & Francis Group, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A β-class carbonic anhydrase (CA, EC 4.2.1.1) was cloned from the genome of the Monogenean platyhelminth Gyrodactylus salaris, a parasite of Atlantic salmon. The new enzyme, GsaCAβ has a significant catalytic activity for the physiological reaction, CO2 + H2O ⇋ HCO3− + H+ with a kcat of 1.1 × 105 s−...