Detailed Pharmacological Characterization of 5-HT1A-Receptor-Mediated [35S]GTPγS Binding in Rat Hippocampal Membranes
5-HT-stimulated [35S]GTPγS binding to rat hippocampal membranes was pharmacologically characterized. Signal/noise ratio or percent increase over basal was optimized with 100 µM GDP, 2 -10 mM MgCl2, and 150 - 200 mM NaCl. However, we preferred the standard condition (20 µM GDP, 5 mM MgCl2, and 100 mM...
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| Main Authors: | , |
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| Format: | Book |
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Elsevier,
2005-01-01T00:00:00Z.
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| Call Number: |
A1234.567 |
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| Copy 1 | Available |