Detection of Wuchereria bancrofti L3 larvae in mosquitoes: a reverse transcriptase PCR assay evaluating infection and infectivity.

• Main Authors: Sandra J Laney (Author), Reda M R Ramzy (Author), Hanan H Helmy (Author), Hoda A Farid (Author), Ameen A Ashour (Author), Gary J Weil (Author), Steven A Williams (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2010-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>Detection of filarial DNA in mosquitoes by PCR cannot differentiate infective mosquitoes from infected mosquitoes. In order to evaluate transmission risk an assay is needed that can specifically detect infective L3 stage parasites. We now report the development of an a...