Determination of single-molecule transport activity of OATP2B1 by measuring the number of transporter molecules using electrophysiological approach

• Main Authors: Kodai Yajima (Author), Takeshi Akiyoshi (Author), Kazuho Sakamoto (Author), Yoshiaki Suzuki (Author), Takayuki Oka (Author), Ayuko Imaoka (Author), Hisao Yamamura (Author), Junko Kurokawa (Author), Hisakazu Ohtani (Author)
• Format: Book
• Published: Elsevier, 2023-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Transporter-mediated clearance is determined by two factors, its single-molecule clearance, and expression level. However, no reliable method has been developed to evaluate them separately. This study aimed to develop a reliable method for evaluating the single-molecule activity of membrane transpor...