Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1

• Main Authors: Raúl López-Fer (Author), Jorge R. Soliz-Rueda (Author), Javier Ávila-Román (Author), Anna Arola-Arnal (Author), Manuel Suárez (Author), Begoña Muguerza (Author), Francisca Isabel Bravo (Author)
• Formato: Libro
• Publicado: MDPI AG, 2021-07-01T00:00:00Z.
• Subjects: article
• Acceso en liña: Connect to this object online.

Summary

The antihypertensive effect of wine lees powder (WLPW) from a Cabernet grape variety was related to its high content in flavanols and anthocyanins compounds. This study investigates the involvement of endothelial-derived factors and SIRT1 in its bioactivity. Spontaneously hypertensive rats (SHR) were orally administered water or WLPW (125 mg/kg bw). Posteriorly, both groups were intraperitoneally administered saline, Nω-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthesis inhibitor, indomethacin, a prostacyclin synthesis inhibitor, or sirtinol, an inhibitor of sirtuins. Blood pressure (BP) was recorded before and 6 h after WLPW administration. In an additional experiment, SHR were administered water or WLPW and endothelial expressions of <i>eNos</i>, <i>Sirt1</i>, <i>Nox4</i>, and <i>Et1</i> were determined. The BP-lowering properties of WLPW were abolished by L-NAME and partially reduced by indomethacin, demonstrating that WLPW antihypertensive effect was mediated by changes in NO availability, although prostacyclin also contributed to this activity. Moreover, BP-lowering effect was reduced by sirtinol, indicating that WLPW decreased BP in a SIRT1-dependent manner. Furthermore, WLPW upregulated <i>eNos</i> and <i>Sirt1</i> and downregulated <i>Nox4</i> and <i>Et1</i> endothelial gene expression. These results evidence the vasoprotective effect of WLPW and show that its antihypertensive effect in SHR is endothelium dependent and mediated by SIRT1.

descrición da copia

10.3390/antiox10071073
2076-3921