In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography

• Main Authors: Yanqiu Gu (Author), Rong Wang (Author), Panpan Chen (Author), Shengnan Li (Author), Xinyi Chai (Author), Chun Chen (Author), Yue Liu (Author), Yan Cao (Author), Diya Lv (Author), Zhanying Hong (Author), Zhenyu Zhu (Author), Yifeng Chai (Author), Yongfang Yuan (Author), Xiaofei Chen (Author)
• Format: Book
• Published: Elsevier, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Cell membrane affinity chromatography has been widely applied in membrane protein (MP)-targeted drug screening and interaction analysis. However, in current methods, the MP sources are derived from cell lines or recombinant protein expression, which are time-consuming for cell culture or purificatio...