Microbiological Assessment of Titanium Plates Coated with PLGA, Chitosan, and/or Meropenem: An In Vitro Study

• Main Authors: Mohammad Al-Qubaisey (Author), Rita Khounganian (Author), Abdulhakim Al-Badah (Author)
• Format: Book
• Published: MDPI AG, 2022-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

This investigation was conducted to evaluate the efficacy of coated titanium plates against gram-positive <i>Staphylococcus aureus</i> (<i>SA</i>) and gram-negative <i>Pseudomonas aeruginosa</i> (<i>PA</i>) using various methods. The Colony-forming unit "CFU" was higher in chitosan (CH) in relation to <i>PA</i> than in poly lactic-co-glycolic acid (PLGA) in relation to <i>SA</i>, followed by meropenem-chitosan "MC" coated plates in relation to <i>PA</i> and <i>SA</i>. A significant difference in the zone of inhibition (ZOI) of <i>SA</i> was determined in MP, MC, and meropenem (MEM). <i>PA</i> was significantly inhibited by MP, MEM, then MC, and the largest ZOI among <i>SA</i> and <i>PA</i> groups were MP coating. Using an MTT assay, MP had the lowest bacterial viability in the <i>SA</i> group, followed by MC and MEM, with no statistically significant difference between the PLGA or CH alone nor the polymers augmented with MEM. Using confocal microscopy, MP-coated plates were seen to have the highest bacterial inhibition, followed by MC, MEM, PLGA, and CH. In the <i>PA</i> group, MP had the highest bacterial inhibition, followed by MEM, MC, CH, and PLGA. The uncoated group presented the lowest inhibition in relation to both <i>SA and PA</i>. Conclusively, coating titanium plates with PLGA or CH with MEM appeared to enhance the antibacterial efficacy as opposed to MEM without polymers through bacterial adhesion inhibition, hindering biofilm formation and preventing bacterial proliferation.

Item Description

10.3390/antibiotics11111565
2079-6382