Conformational Equilibrium of NADPH-Cytochrome P450 Oxidoreductase Is Essential for Heme Oxygenase Reaction

• Main Authors: Masakazu Sugishima (Author), Junichi Taira (Author), Tatsuya Sagara (Author), Ryota Nakao (Author), Hideaki Sato (Author), Masato Noguchi (Author), Keiichi Fukuyama (Author), Ken Yamamoto (Author), Takuo Yasunaga (Author), Hiroshi Sakamoto (Author)
• Format: Book
• Published: MDPI AG, 2020-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Heme oxygenase (HO) catalyzes heme degradation using electrons supplied by NADPH-cytochrome P450 oxidoreductase (CPR). Electrons from NADPH flow first to FAD, then to FMN, and finally to the heme in the redox partner. Previous biophysical analyses suggest the presence of a dynamic equilibrium betwee...