Removal of HIV DNA by CRISPR from Patient Blood Engrafts in Humanized Mice

• Main Authors: Ramona Bella (Author), Rafal Kaminski (Author), Pietro Mancuso (Author), Won-Bin Young (Author), Chen Chen (Author), Rahsan Sariyer (Author), Tracy Fischer (Author), Shohreh Amini (Author), Pasquale Ferrante (Author), Jeffrey M. Jacobson (Author), Fatah Kashanchi (Author), Kamel Khalili (Author)
• Format: Book
• Published: Elsevier, 2018-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We used NOD/SCID mice, also known as NRG, to assess the ability of lentivirus-mediated intravenous delivery of CRISPR in editing the HIV-1 genome from the circulating PBMC engrafts, some of which homed within several animal solid tissues. Lentivirus-mediated delivery of a multiplex of guide RNAs acc...