Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene Targeting

• Үндсэн зохиолчид: Carsten T. Charlesworth (Зохиогч), Joab Camarena (Зохиогч), M. Kyle Cromer (Зохиогч), Sriram Vaidyanathan (Зохиогч), Rasmus O. Bak (Зохиогч), Jason M. Carte (Зохиогч), Jason Potter (Зохиогч), Daniel P. Dever (Зохиогч), Matthew H. Porteus (Зохиогч)
• Формат: Ном
• Хэвлэсэн: Elsevier, 2018-09-01T00:00:00Z.
• Нөхцлүүд: article
• Онлайн хандалт: Connect to this object online.

Engineered nuclease-mediated gene targeting through homologous recombination (HR) in hematopoietic stem and progenitor cells (HSPCs) has the potential to treat a variety of genetic hematologic and immunologic disorders. Here, we identify critical parameters to reproducibly achieve high frequencies o...