Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293

• Main Authors: Ly Porosk (Author), Jekaterina Nebogatova (Author), Heleri Heike Härk (Author), Birgit Vunk (Author), Piret Arukuusk (Author), Urve Toots (Author), Mart Ustav (Author), Ülo Langel (Author), Kaido Kurrikoff (Author)
• Format: Book
• Published: MDPI AG, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Therapeutic proteins are currently at the apex of innovation in pharmaceutical medicine. However, their industrial production is technically challenging and improved methods for transient transfection of mammalian cell cultures are necessary. We aimed to find a fast, microliter-scale transfection as...