Comparison of four DNA extraction and three preservation protocols for the molecular detection and quantification of soil-transmitted helminths in stool.

• Main Authors: Mio Ayana (Author), Piet Cools (Author), Zeleke Mekonnen (Author), Abdissa Biruksew (Author), Daniel Dana (Author), Nour Rashwan (Author), Roger Prichard (Author), Johnny Vlaminck (Author), Jaco J Verweij (Author), Bruno Levecke (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:A DNA extraction and preservation protocol that yields sufficient and qualitative DNA is pivotal for the success of any nucleic acid amplification test (NAAT), but it still poses a challenge for soil-transmitted helminths (STHs), including Ascaris lumbricoides, Trichuris trichiura and the...