Subgenomic particles in rAAV vectors result from DNA lesion/break and non-homologous end joining of vector genomes

• Main Authors: Junping Zhang (Author), Ping Guo (Author), Xiangping Yu (Author), Dylan A. Frabutt (Author), Anh K. Lam (Author), Patrick L. Mulcrone (Author), Matthew Chrzanowski (Author), Jenni Firrman (Author), Derek Pouchnik (Author), Nianli Sang (Author), Yong Diao (Author), Roland W. Herzog (Author), Weidong Xiao (Author)
• Format: Book
• Published: Elsevier, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Recombinant adeno-associated virus (rAAV) vectors have been developed for therapeutic treatment of genetic diseases. Current rAAV vectors administered to affected individuals often contain vector DNA-related contaminants. Here we present a thorough molecular analysis of the configuration of non-stan...