Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scope

• Main Authors: Lukas Villiger (Author), Lukas Schmidheini (Author), Nicolas Mathis (Author), Tanja Rothgangl (Author), Kim Marquart (Author), Gerald Schwank (Author)
• Format: Book
• Published: Elsevier, 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Base editors are RNA-guided deaminases that enable site-specific nucleotide transitions. The targeting scope of these Cas-deaminase fusion proteins critically depends on the availability of a protospacer adjacent motif (PAM) at the target locus and is limited to a window within the CRISPR-Cas R-loop...