The impact of different DNA extraction methods on the analysis of microbial diversity of oral saliva from healthy youths by polymerase chain reaction-denaturing gradient gel electrophoresis

• Glavni autori: Ming Chen (Autor), Bu-Ling Wu (Autor), Ting Chen (Autor), Zhao Liu (Autor), Zi-Long Deng (Autor), Ling Peng (Autor)
• Format: Knjiga
• Izdano: Elsevier, 2016-03-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

Background/purpose: Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), as a conventional molecular technique, was utilized to analyze the diversity of oral microbiota. However, studies found that the results of PCR-DGGE were affected by the DNA isolation method. This study...