The impact of different DNA extraction methods on the analysis of microbial diversity of oral saliva from healthy youths by polymerase chain reaction-denaturing gradient gel electrophoresis

• Main Authors: Ming Chen (Author), Bu-Ling Wu (Author), Ting Chen (Author), Zhao Liu (Author), Zi-Long Deng (Author), Ling Peng (Author)
• Format: Book
• Published: Elsevier, 2016-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background/purpose: Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), as a conventional molecular technique, was utilized to analyze the diversity of oral microbiota. However, studies found that the results of PCR-DGGE were affected by the DNA isolation method. This study...