Characterization of ESBL (SHV-12) producing clinical isolate of <it>Enterobacter aerogenes </it>from a tertiary care hospital in Nigeria

• Main Authors: Budak Fatma (Author), Kolayli Fetiye (Author), Torol Sinem (Author), Fashae Kayode (Author), Kasap Murat (Author), Vahaboglu Haluk (Author)
• Format: Book
• Published: BMC, 2010-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p>We studied the beta-lactamases of an <it>E. aerogenes </it>isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.</p> <p>Methods</p> <p>Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.</p> <p>Results</p> <p>By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an <it>E. coli </it>host. Sequence analysis of the recombinant plasmid revealed <it>bla</it>_SHV-12associated to the insertion sequence, IS<it>26</it>.</p> <p>Conclusion</p> <p>This is the first study demonstrated the occurrence of SHV-12 in Nigeria.</p>

Item Description

10.1186/1476-0711-9-1
1476-0711