Utility of prospective step sections in diagnostic skin histopathology for small biopsies

• Main Authors: G Kattel (Author), AK Sinha (Author), S Agrawal (Author)
• Format: Book
• Published: Association of Clinical Pathologists of Nepal, 2014-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p class="Default"><strong style="font-size: 10px;">Background: </strong><span style="font-size: 10px;">Small skin biopsies have a cosmetic advantage and prospective step sections could potentially improve turnaround time without compromising diagnostic information.</span></p> <p class="Pa19"><span>The study aimed to examine the use of prospective step sections of small skin biopsies and its effect on histopathological diagnosis and turnaround time. </span></p> <p class="Pa19"><strong><span>Materials and Methods: </span></strong><span>This was a hospital based cross-sectional study at Department of Pathology and Department of Dermatology, BPKIHS from June 2011- June 2012. </span></p> <p class="Pa19"><span>Diagnoses /comment on three levels of prospectively taken 3mm biopsies were compared with those of 5mm biopsies taken from the same/similar lesion from 100 patients. Additional sections from 5mm biopsies were taken retrospectively. Percentage, proportion, mean, standard deviation, diagnostic sensitivity, kappa statistics and paired sample t- test were used as statistical tools. </span></p> <p class="Pa19"><strong><span>Results: </span></strong><span>Of 100 cases, 80 were diagnosed using 5mm biopsies while 73 were diagnosed using 3mm biopsies. On additional step sections of 3mm biopsies, 3 more cases were diagnosed. When compared with 5mm biopsies, the sensitivity of the 3mm biopsy rose from 90% to 93.8% after additional sections while the measure of agreement rose from 0.751 to 0.826.Mean turnaround time for prospectively sectioned 3mm biopsies was 2.56 days and that of retrospectively sectioned 5 mm biopsies was 4.64 days with their difference being statistically significant. </span></p> <p class="Pa19"><strong><span>Conclusion: </span></strong><span>A statistically significant decrease in turnaround time and an increase in sensitivity and agreement after step sections elucidates the utility of prospective step sectioned 3 mm biopsies in diagnostic skin histopathology. </span></p> <p class="Pa19"><span>DOI:<span>&nbsp;</span><a href="http://dx.doi.org/10.3126/jpn.v4i7.10298">http://dx.doi.org/10.3126/jpn.v4i7.10298</a> </span></p> <p><span>Journal of Pathology of Nepal (2014) Vol.<span>&nbsp;</span><strong>4</strong>, 552-559 &nbsp;</span></p>

Item Description

2091-0797
2091-0908
10.3126/jpn.v4i7.10298