Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus

• Main Authors: Junya Ichikawa (Author), Ryuji X Yamada (Author), Rieko Muramatsu (Author), Yuji Ikegaya (Author), Norio Matsuki (Author), Ryuta Koyama (Author)
• Format: Book
• Published: Elsevier, 2007-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Although primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as lon...