<i>Aronia melanocarpa</i> Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells

• Main Authors: Bong-Keun Jang (Author), Jin-Woo Lee (Author), Hyun Choi (Author), Sung-Vin Yim (Author)
• Format: Book
• Published: MDPI AG, 2020-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

To demonstrate the anti-inflammatory activity of <i>Aronia melanocarpa</i> fruit extract, human bronchial epithelial cells (BEAS-2B) were treated with lipopolysaccharide (LPS) and the effects of aronia bioactive fraction (ABF^®), anthocyanin enriched extract from the fruit of <i>A. melanocarpa,</i> were evaluated. Following pretreatment with ABF^® at 10-25 µg /mL, BEAS-2B cells were exposed to LPS and the expression of inflammatory mediators (tumor necrosis factor [TNF]-α, interleukin [IL]-6, IL-8, regulated upon activation, normal T cell expressed and presumably secreted [RANTES], IL-1β, cyclooxygenase-2 [COX-2], and inducible nitric oxide synthase [iNOS]) was analyzed. In LPS-stimulated BEAS-2B cells, ABF^® pretreatment significantly decreased the mRNA expression of TNF-α, IL-6, IL-8, RANTES, IL-1β, and COX-2 at doses of 10 and 25 µg/mL. ABF^® also attenuated the secretion of TNF- α, IL-6, IL-8, and RANTES protein, as demonstrated by enzyme linked immunosorbent assay. Western blot analyses revealed the decreased expression of COX-2 and iNOS following ABF^® treatment. ROS production was decreased, and the cell cycle was arrested at the G0/G1 and S phases following ABF^® pretreatment. Our results suggest that ABF^® may have potential as a nutraceutical agent for the suppression of airway inflammation.

Item Description

10.3390/antiox9090816
2076-3921