Single cell phototransfection of mRNAs encoding SARS-CoV2 spike and nucleocapsid into human astrocytes results in RNA dependent translation interference

• Main Authors: Hyun-Bum Kim (Author), Quentin Brosseau (Author), Julia Radzio (Author), Jinhui Wang (Author), Hiromi Muramatsu (Author), Da Kuang (Author), M. Sean Grady (Author), H. Isaac Chen (Author), John A. Wolf (Author), Alexandra V. Ulyanova (Author), Tamas Bartfai (Author), Junhyong Kim (Author), Norbert Pardi (Author), Jai-Yoon Sul (Author), Paulo Arratia (Author), James Eberwine (Author)
• Format: Book
• Published: Frontiers Media S.A., 2024-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Multi-RNA co-transfection is starting to be employed to stimulate immune responses to SARS-CoV-2 viral infection. While there are good reasons to utilize such an approach, there is little background on whether there are synergistic RNA-dependent cellular effects. To address this issue, we use transc...