Deciphering the Path of <i>S-nitrosation</i> of Human Thioredoxin: Evidence of an Internal NO Transfer and Implication for the Cellular Responses to NO
Nitric oxide (NO) is a free radical with a signaling capacity. Its cellular functions are achieved mainly through <i>S-nitrosation</i> where thioredoxin (hTrx) is pivotal in the S-transnitrosation to specific cellular targets. In this study, we use NMR spectroscopy and mass spectrometry...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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MDPI AG,
2022-06-01T00:00:00Z.
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| Summary: | Nitric oxide (NO) is a free radical with a signaling capacity. Its cellular functions are achieved mainly through <i>S-nitrosation</i> where thioredoxin (hTrx) is pivotal in the S-transnitrosation to specific cellular targets. In this study, we use NMR spectroscopy and mass spectrometry to follow the mechanism of S-(trans)nitrosation of hTrx. We describe a site-specific path for <i>S-nitrosation</i> by measuring the reactivity of each of the 5 cysteines of hTrx using cysteine mutants. We showed the interdependence of the three cysteines in the nitrosative site. C73 is the most reactive and is responsible for all S-transnitrosation to other cellular targets. We observed NO internal transfers leading to C62 <i>S-nitrosation</i>, which serves as a storage site for NO. C69-SNO only forms under nitrosative stress, leading to hTrx nuclear translocation. |
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| Item Description: | 10.3390/antiox11071236 2076-3921 |