Proximity Ligation Assays for In Situ Detection of Innate Immune Activation: Focus on In Vitro-Transcribed mRNA

• Main Authors: Emmeline L. Blanchard (Author), Kristin H. Loomis (Author), Sushma M. Bhosle (Author), Daryll Vanover (Author), Patrick Baumhof (Author), Bruno Pitard (Author), Chiara Zurla (Author), Philip J. Santangelo (Author)
• Format: Book
• Published: Elsevier, 2019-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The characterization of innate immune activation is crucial for vaccine and therapeutic development, including RNA-based vaccines, a promising approach. Current measurement methods quantify type I interferon and inflammatory cytokine production, but they do not allow for the isolation of individual...