Engineering miniature CRISPR-Cas Un1Cas12f1 for efficient base editing

• Main Authors: Yueer Hu (Author), Linxiao Han (Author), Qiqin Mo (Author), Zengming Du (Author), Wei Jiang (Author), Xia Wu (Author), Jing Zheng (Author), Xiao Xiao (Author), Yadong Sun (Author), Hanhui Ma (Author)
• Format: Book
• Published: Elsevier, 2024-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Adeno-associated virus (AAV) is a relatively safe and efficient vector for gene therapy. However, due to its 4.7-kb limit of cargo, SpCas9-mediated base editors cannot be packaged into a single AAV vector, which hinders their clinical application. The development of efficient miniature base editors...