A panel of Trypanosoma brucei strains tagged with blue and red-shifted luciferases for bioluminescent imaging in murine infection models.

• Main Authors: Nick Van Reet (Author), Hélène Van de Vyver (Author), Patient Pati Pyana (Author), Anne Marie Van der Linden (Author), Philippe Büscher (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2014-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:Genetic engineering with luciferase reporter genes allows monitoring Trypanosoma brucei (T.b.) infections in mice by in vivo bioluminescence imaging (BLI). Until recently, luminescent T.b. models were based on Renilla luciferase (RLuc) activity. Our study aimed at evaluating red-shifted l...