Evaluation of 124I-JS001 for hPD1 immuno-PET imaging using sarcoma cell homografts in humanized mice
JS001 (toripalimab) is a humanized IgG monoclonal antibody which strongly inhibits programmed cell death protein 1 (PD1). In this study, we used a different iodine isotype (nat/124/125I) to label JS001 probes to target the human PD1 (hPD1) antigen. In vitro, the half maximal effective concentration...
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Elsevier,
2020-07-01T00:00:00Z.
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| 001 | doaj_cfd2c39e4ddf4dce80d534baafe6310c | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Haifeng Huang |e author |
| 700 | 1 | 0 | |a Hua Zhu |e author |
| 700 | 1 | 0 | |a Quan Xie |e author |
| 700 | 1 | 0 | |a Xiaobin Tian |e author |
| 700 | 1 | 0 | |a Xianteng Yang |e author |
| 700 | 1 | 0 | |a Fan Feng |e author |
| 700 | 1 | 0 | |a Qiyu Jiang |e author |
| 700 | 1 | 0 | |a Xinan Sheng |e author |
| 700 | 1 | 0 | |a Zhi Yang |e author |
| 245 | 0 | 0 | |a Evaluation of 124I-JS001 for hPD1 immuno-PET imaging using sarcoma cell homografts in humanized mice |
| 260 | |b Elsevier, |c 2020-07-01T00:00:00Z. | ||
| 500 | |a 2211-3835 | ||
| 500 | |a 10.1016/j.apsb.2020.02.004 | ||
| 520 | |a JS001 (toripalimab) is a humanized IgG monoclonal antibody which strongly inhibits programmed cell death protein 1 (PD1). In this study, we used a different iodine isotype (nat/124/125I) to label JS001 probes to target the human PD1 (hPD1) antigen. In vitro, the half maximal effective concentration (EC50) value of natI-JS001 did not significantly differ from that of JS001. The uptake of 125I-JS001 by activated T cells was 5.63 times higher than that by nonactivated T cells after 2 h of incubation. The binding affinity of 125I-JS001 to T cells of different lineages after phytohemagglutinin (PHA) stimulation reached 4.26 nmol/L. Humanized PD1 C57BL/6 mice bearing mouse sarcoma S180 cell tumors were validated for immuno-positron emission tomography (immuno-PET) imaging. Pathological staining was used to assess the expression of PD1 in tumor tissues. The homologous 124I-human IgG (124I-hIgG) group or blocking group was used as a control group. Immuno-PET imaging showed that the uptake in the tumor area of the 124I-JS001 group at different time points was significantly higher than that of the blocking group or the 124I-hIgG group in the humanized PD1 mouse model. Taken together, these results suggest that this radiotracer has potential for noninvasive monitoring and directing tumor-specific personalized immunotherapy in PD1-positive tumors. | ||
| 546 | |a EN | ||
| 690 | |a Immunotherapy | ||
| 690 | |a JS001 | ||
| 690 | |a Toripalimab | ||
| 690 | |a Programmed cell death protein 1 | ||
| 690 | |a Immuno-PET imaging | ||
| 690 | |a Iodine isotopes | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Acta Pharmaceutica Sinica B, Vol 10, Iss 7, Pp 1321-1330 (2020) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2211383519310214 | |
| 787 | 0 | |n https://doaj.org/toc/2211-3835 | |
| 856 | 4 | 1 | |u https://doaj.org/article/cfd2c39e4ddf4dce80d534baafe6310c |z Connect to this object online. |