NAb-seq: an accurate, rapid, and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cells

• Main Authors: Hema Preethi Subas Satish (Author), Kathleen Zeglinski (Author), Rachel T. Uren (Author), Stephen L. Nutt (Author), Matthew E. Ritchie (Author), Quentin Gouil (Author), Ruth M. Kluck (Author)
• Format: Book
• Published: Taylor & Francis Group, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Despite their common use in research, monoclonal antibodies are currently not systematically sequenced. This can lead to issues with reproducibility and the occasional loss of antibodies with loss of cell lines. Hybridoma cell lines have been the primary means of generating monoclonal antibodies fro...