Despite low viral titer in saliva samples, Sanger-based SARS-CoV-2 spike gene sequencing is highly applicable for the variant identification

• Main Authors: Ko Ko (Author), Kazuaki Takahashi (Author), Noriaki Ito (Author), Aya Sugiyama (Author), Shintaro Nagashima (Author), Kei Miwata (Author), Yoshihiro Kitahara (Author), Mafumi Okimoto (Author), Serge Ouoba (Author), Golda Ataa Akuffo (Author), Bunthen E (Author), Tomoyuki Akita (Author), Toshiro Takafuta (Author), Junko Tanaka (Author)
• Format: Book
• Published: BMC, 2023-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background This study aimed to compare the performance of Sanger-based SARS-CoV-2 spike gene sequencing and Next Generation Sequencing (NGS)-based full-genome sequencing for variant identification in saliva samples with low viral titer. Methods Using 241 stocked saliva samples collected fro...