A Liquid Chromatography with Tandem Mass Spectrometry-Based Proteomic Analysis of Primary Cultured Cells and Subcultured Cells Using Mouse Adipose-Derived Mesenchymal Stem Cells

• Main Authors: Yoshiki Nakashima (Author), Saifun Nahar (Author), Chika Miyagi-Shiohira (Author), Takao Kinjo (Author), Naoya Kobayashi (Author), Issei Saitoh (Author), Masami Watanabe (Author), Jiro Fujita (Author), Hirofumi Noguchi (Author)
• Format: Book
• Published: Hindawi Limited, 2019-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Adipose-derived mesenchymal stem cells (MSC-ATs) are representative cell sources for cell therapy. However, how cell stress resulting from passage influences the MSC-AT protein expression has been unclear. In this study, a protein expression analysis was performed by liquid chromatography with tande...