Elucidating the Mechanism of Agrimonolide in Treating Colon Cancer Based on Network Pharmacology
Lei Yu, Yun Gai Department of Oncology I, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai City, People's Republic of ChinaCorrespondence: Yun Gai, Department of Oncology I, Seventh People's Hospital of Shanghai University of Traditional Chine...
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Dove Medical Press,
2023-07-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
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| 001 | doaj_d36540e8340a4f9aacd8f5db2bb4b6e8 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Yu L |e author |
| 700 | 1 | 0 | |a Gai Y |e author |
| 245 | 0 | 0 | |a Elucidating the Mechanism of Agrimonolide in Treating Colon Cancer Based on Network Pharmacology |
| 260 | |b Dove Medical Press, |c 2023-07-01T00:00:00Z. | ||
| 500 | |a 1177-8881 | ||
| 520 | |a Lei Yu, Yun Gai Department of Oncology I, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai City, People's Republic of ChinaCorrespondence: Yun Gai, Department of Oncology I, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, No. 358, DaTong Road, PuDong New Area, Shanghai City, 200137, People's Republic of China, Tel +86- 13917898986, Email gaiyun_sh7@yeah.netPurpose: This study reported the efficacy and underlying mechanism of agrimonolide (AM) in treating colon cancer.Methods: Colon cancer-AM-related targets were screened from online database. AM targets for colon cancer were identified by Venn diagram. Main molecular function, biological process, cellular component and pathways associated with AM targets for colon cancer were analyzed by GO and KEGG enrichment analysis. Relationship of the 10 core targets of AM for colon cancer with the top 15 BP and KEGG pathways was analyzed by Cytoscape software. A "component-target-pathway" network was constructed to select the hub genes of AM for colon cancer. AM effects on colon cancer cell viability, proliferation, invasion, migration and apoptosis were researched by CCK-8, colony formation, Transwell invasion, wound healing and flow cytometry assays. Tumor-bearing nude mice models were constructed and given AM treatment. Hub gene expression in cells/tissues was detected by Western blot.Results: A total of 107 targets were selected as AM targets for colon cancer. The 10 core targets were related to the top 15 biological process terms and KEGG pathways. PI3K, AKT and mTOR were selected as the hub genes of AM for colon cancer. AM weakened colon cell proliferation, invasion, migration and apoptosis inhibition, and suppressed colon cell in vivo growth. AM up-regulated Caspase-3 and BAX proteins, down-regulated C-Myc, Cyclin D1 and BCL-2 proteins, and inactivated the PI3K/AKT/mTOR pathway both in vitro and in vivo.Conclusion: AM suppressed colon cancer progression through inactivating the PI3K/AKT/mTOR pathway. It may be useful for colon cancer treatment.Keywords: colon cancer, agrimonolide, network pharmacology, PI3K/AKT/mTOR pathway, in vivo | ||
| 546 | |a EN | ||
| 690 | |a colon cancer | ||
| 690 | |a agrimonolide | ||
| 690 | |a network pharmacology | ||
| 690 | |a pi3k/akt/mtor pathway | ||
| 690 | |a in vivo | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Drug Design, Development and Therapy, Vol Volume 17, Pp 2209-2222 (2023) | |
| 787 | 0 | |n https://www.dovepress.com/elucidating-the-mechanism-of-agrimonolide-in-treating-colon-cancer-bas-peer-reviewed-fulltext-article-DDDT | |
| 787 | 0 | |n https://doaj.org/toc/1177-8881 | |
| 856 | 4 | 1 | |u https://doaj.org/article/d36540e8340a4f9aacd8f5db2bb4b6e8 |z Connect to this object online. |