Vitamin C in the Presence of Sub-Inhibitory Concentration of Aminoglycosides and Fluoroquinolones Alters <i>Proteus mirabilis</i> Biofilm Inhibitory Rate
Vitamin C has antimicrobial activity and is often used as an oral supplement accompanying antibiotic treatment in urinary tract infections (UTI). <i>Proteus mirabilis</i> is the third common species responsible for UTIs that are mostly treated with fluoroquinolones or aminoglycosides. Tr...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2019-08-01T00:00:00Z.
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| Summary: | Vitamin C has antimicrobial activity and is often used as an oral supplement accompanying antibiotic treatment in urinary tract infections (UTI). <i>Proteus mirabilis</i> is the third common species responsible for UTIs that are mostly treated with fluoroquinolones or aminoglycosides. Treatment of the UTI caused by <i>P. mirabilis</i> is problematic due to the ability to form biofilm on the urinary catheters. The aim of the study was to evaluate the influence of ascorbic acid in combination with antibiotics on <i>P. mirabilis</i> abilities to form biofilm. The susceptibility of <i>P. mirabilis</i> reference strain ATCC<sup>®</sup> 29906™ and four clinical strains isolated from the urine samples of patients with urinary catheter were evaluated according to EUCAST recommendations. The influence of ascorbic acid (0.4 mg × mL<sup>−1</sup>) in combination with antibiotics on biofilm formation was evaluated spectrophotometrically. Aminoglycosides at sub-inhibitory concentrations more successfully limited biofilm formation by <i>P. mirabilis</i> strains without ascorbic acid addition. Inhibition rate differences at the lowest concentrations of gentamicin and amikacin were statistically significant (<i>p</i> ≤ 0.05). Ascorbic acid addition to the culture medium limited the inhibitory effect of fluoroquinolones, facilitating biofilm formation by <i>P. mirabilis</i> strains. The addition of ascorbic acid during aminoglycosides therapy may disturb treatment of urinary tract infections related to the presence of <i>P. mirabilis</i> biofilm. |
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| Item Description: | 2079-6382 10.3390/antibiotics8030116 |