Three-Dimensional Gastrointestinal Organoid Culture in Combination with Nerves or Fibroblasts: A Method to Characterize the Gastrointestinal Stem Cell Niche

• Main Authors: Agnieszka Pastuła (Author), Moritz Middelhoff (Author), Anna Brandtner (Author), Moritz Tobiasch (Author), Bettina Höhl (Author), Andreas H. Nuber (Author), Ihsan Ekin Demir (Author), Steffi Neupert (Author), Patrick Kollmann (Author), Gemma Mazzuoli-Weber (Author), Michael Quante (Author)
• Format: Book
• Published: Hindawi Limited, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The gastrointestinal epithelium is characterized by a high turnover of cells and intestinal stem cells predominantly reside at the bottom of crypts and their progeny serve to maintain normal intestinal homeostasis. Accumulating evidence demonstrates the pivotal role of a niche surrounding intestinal...