Scalable and DiI-compatible optical clearance of the mammalian brain

• Main Authors: Bing eHou (Author), Dan eZhang (Author), Shan eZhao (Author), Mengping eWei (Author), Zaifu eYang (Author), Shaoxia eWang (Author), Jiarui eWang (Author), Xin eZhang (Author), Bing eLiu (Author), Lingzhong eFan (Author), Yang eLi (Author), Zilong eQiu (Author), Chen eZhang (Author), Tianzi eJiang (Author)
• Format: Book
• Published: Frontiers Media S.A., 2015-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Efficient optical clearance is fundamental for whole brain imaging. In particular, clearance of the brain without membrane damage is required for the imaging of lipophilic tracer-labeled neural tracts. Relying on an ascending gradient of fructose solutions, SeeDB can achieve sufficient transparency...